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dc.contributor.authorEpperson, Erin
dc.contributor.authorKirby, John
dc.date.accessioned2007-03-05T19:05:00Z
dc.date.available2007-03-05T19:05:00Z
dc.date.issued2006-12-15
dc.identifier.urihttp://hdl.handle.net/1853/13580
dc.description.abstractMyxococcus xanthus is a Gram negative soil bacterium that utilizes eight chemosensory systems of interacting proteins, most of which are homologous to the E.coli chemotaxis proteins, in order to regulate its two types of gliding motility (A and S), predation of other organisms, and multicellular fruiting body formation. The M. xanthus Che7 system, which has not been thoroughly characterized to date, is of specific interest because it is thought to regulate A motility through exopolysaccharide (EPS) production, contain a completely cytoplasmic Mcp (methyl-accepting chemotaxis protein), and have two functioning non-chemotaxis proteins: Cpc7 and Des7. In this study, we explored the protein-protein interactions of the Che7 system of M. xanthus through yeast two-hybrid and beta-galactosidase assays. In addition to the expected interactions between the Che7 chemotaxis homologs, we found that Cpc7 interacted with both Des7 and Mcp7. This suggests that Cpc7 is cytoplasmic and that several possibilities for the mechanism of signal transduction in the Che7 system of M. xanthus exist. From these results, we propose a new protein interaction model for the Che7 chemosensory two-component signal transduction system.en
dc.format.extent150159 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.publisherGeorgia Institute of Technologyen
dc.subjectMyxococcus xanthus
dc.subjectChemosensory systems
dc.subjectChe7 system
dc.subjectSignal transduction
dc.subjectProtein interaction models
dc.titleCharacterization of the Che7 System of Myxococcus xanthusen
dc.typeUndergraduate Thesisen
dc.contributor.departmentBiology
dc.description.advisorJohn Kirby


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