• Login
    View Item 
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Bone Marrow-Derived Mesenchymal Stem Cells As an Alternate Donor Cell Source for Transplantation in Tissue-Engineered Constructs After Traumatic Brain Injury

    Thumbnail
    View/Open
    Irons_Hillary_R_200708_phd.pdf (7.675Mb)
    Date
    2007-07-09
    Author
    Irons, Hillary Rose
    Metadata
    Show full item record
    Abstract
    The incidence and long-term effects of traumatic brain injury (TBI) make it a major healthcare and socioeconomic concern. Cell transplantation may be an alternative therapy option to target prolonged neurological deficits; however, safety and efficacy of the cells must be determined. Bone marrow-derived mesenchymal stem cells (MSCs) are an accessible and expandable cell source which circumvent the many of the accessibility and ethical concerns associated with fetal tissues. A major impediment to recent clinical trials for cell therapies in the central nervous system has been the lack of consistency in functional recovery where some patients receive great benefits while others experience little, if any, effect (Watts and Dunnett 2000; Lindvall and Bjorklund 2004). There are many possible explanations for this patient-to-patient variability including genetic and environmental factors, surgical techniques, and donor cell variability. Of these, the most easily addressable is to increase the reproducibility of donor cells by standardizing the isolation and pre-transplantation protocols, which is the central goal of this dissertation. First, we present an animal study in which transplants of MSCs and neural stem cells (NSCs) were given to brain-injured mice, however, the efficacy of the treatment had high variability between individual subjects. Second, we designed a method to produce MSC-spheres and characterize them in vitro. Last, we employed an in vitro 3-D culture testbed as a pre-transplant injury model to assess the effects of the MSC-spheres on neural cells. The electrophysiological function of the uninjured testbed was assessed, and then MSC-spheres were injected into the testbed and apoptosis of the host cells were measured. The results of this study contribute to our understanding of how extracellular context may influence MSC-spheres and develop MSCs as a donor cell source for transplantation.
    URI
    http://hdl.handle.net/1853/16168
    Collections
    • Department of Biomedical Engineering Theses and Dissertations [575]
    • Georgia Tech Theses and Dissertations [23877]

    Browse

    All of SMARTechCommunities & CollectionsDatesAuthorsTitlesSubjectsTypesThis CollectionDatesAuthorsTitlesSubjectsTypes

    My SMARTech

    Login

    Statistics

    View Usage StatisticsView Google Analytics Statistics
    facebook instagram twitter youtube
    • My Account
    • Contact us
    • Directory
    • Campus Map
    • Support/Give
    • Library Accessibility
      • About SMARTech
      • SMARTech Terms of Use
    Georgia Tech Library266 4th Street NW, Atlanta, GA 30332
    404.894.4500
    • Emergency Information
    • Legal and Privacy Information
    • Human Trafficking Notice
    • Accessibility
    • Accountability
    • Accreditation
    • Employment
    © 2020 Georgia Institute of Technology