Scanning Mass Spectrometry (SMS) Probe
MetadataShow full item record
Peter Kottke described the SMS probe, a tool currently under development for mass spectrometric imaging of live biological samples in vitro. In order to obtain spatially resolved images of chemical species distributions in vitro, an ion source is needed that samples from a small volume of liquid. The SMS probe is based on a novel approach to electrospray ionization (reverse-Taylor-cone electrospray), which requires a reversal of orientation of conventional electrospray ionization (ESI) sampling. Electrospray ionization mass spectrometry (ESI-MS) has the advantage of providing a means for transferring large biological molecules directly from solution into the gas phase under atmospheric pressure. The unique advantages of the reverse-Taylor-cone ionization concept are the minimum and highly localized (in space) sample volume (i.e., high resolution) that is being probed and, most importantly, its unique suitability for use in scanning probe chemical imaging applications, including imaging biological samples under physiological conditions (i.e., in aqueous solution).
- Nano@Tech Lecture Series