A Biological Engineered Approach to Enabling Adult Stem Cell-Based Biomedicine
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Adult stem cells (ASCs) have potential application in several areas of biomedicine, including cell therapy, gene therapy, and tissue engineering. However, development of ASCs for this purpose has been precluded by difficulty producing them in sufficient number and, consequently, limited knowledge of their properties. We developed a biological engineered approach for ASC production that is based on suppression of asymmetric cell kinetics (SACK). The SACK method effectively expands ASCs from diverse mammalian tissues. Investigations with the expanded ASCs confirmed that ASCs exhibit adifferentiation under conditions that induce active differentiation of their progeny cells. ASC adifferentiation may be the key to discovery of markers that uniquely identify ASCs, another critical tool needed to enable ASC-based biomedicine.