Enrichment of Adipose-derived Mesenchymal Stem Cells Using Resveratrol
Erdman, Christopher Paul
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Mesenchymal stem cells (MSCs) hold great promise for the application of tissue engineering to orthopedic problems such as critical-size defects. Cell sorting has indicated a benefit to enriching the MSC population, but is undesired for clinical applications. Pharmacological enrichment utilizing adipogenic inhibitors such as resveratrol represent a clinically viable alternative. Cells were isolated from rats, and treated with differentiation media to verify multipotency, and were then treated with 0, 12.5, and 25 µM resveratrol in growth media and osteogenic media. MSC and osteoprogenitor (OPC) populations were measured using flow cytometry and OPC quality was assessed with osteocalcin production, osteoprotegerin production, and alkaline phosphatase activity. Resveratrol increased the population percent and cell number of MSCs in both growth and osteogenic media, but only increased the number of OPCs in growth media. In both media types resveratrol increased alkaline phosphatase activity and osteocalcin levels. Resveratrol enriches ADMSCs for mesenchymal stem cells and osteoprogenitors.