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dc.contributor.authorSun, Xueguangen_US
dc.contributor.authorZhulin, Igoren_US
dc.contributor.authorWartell, Roger M.en_US
dc.date.accessioned2009-10-09T18:05:30Z
dc.date.available2009-10-09T18:05:30Z
dc.date.issued2002
dc.identifier.citationSun, X., I. Zhulin, and R. M. Wartell, "Predicted structure and phyletic distribution of the RNA binding protein Hfq," Nucleic Acids Research, 30:3693-3700en_US
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/1853/30438
dc.description© 2002 Oxford University Press. The definitive version is available online at: http://nar.oxfordjournals.org/en_US
dc.descriptionDOI: 10.1093/nar/gkf508en_US
dc.description.abstractHfq, a bacterial RNA-binding protein, was recently shown to contain the Sm1 motif, a characteristic of Sm and LSm proteins that function in RNA processing events in archaea and eukaryotes. In this report, comparative structural modeling was used to predict a three-dimensional structure of the Hfq core sequence. The predicted structure aligns with most major features of the Methanobacterium thermoautotrophicum LSm protein structure. Conserved residues in Hfq are positioned at the same structural locations responsible for subunit assembly and RNA interaction in Sm proteins. A highly conserved portion of Hfq assumes a structural fold similar to the Sm2 motif of Sm proteins. The evolution of the Hfq protein was explored by conducting a BLAST search of microbial genomes followed by phylogenetic analysis. Approximately half of the 140 complete or nearly complete genomes examined contain at least one gene coding for Hfq. The presence or absence of Hfq closely followed major bacterial clades. It is absent from high-level clades and present in the ancient Thermotogales-Aquificales clade and all proteobacteria except for those that have undergone major reduction in genome size. Residues at three positions in Hfq form signatures for the beta/gamma proteobacteria, alpha proteobacteria and low GC Gram-positive bacteria groups.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectHfqen_US
dc.subjectProtein structureen_US
dc.subjectRNA bindingen_US
dc.subjectMicrobial genomesen_US
dc.subjectCladesen_US
dc.titlePredicted structure and phyletic distribution of the RNA-binding protein Hfqen_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.publisher.originalOxford University Pressen_US
dc.identifier.doi10.1093/nar/gkf508


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