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dc.contributor.authorMurga, Matildeen_US
dc.contributor.authorJaco, Isabelen_US
dc.contributor.authorFan, Yuhongen_US
dc.contributor.authorSoria, Rebecaen_US
dc.contributor.authorMartinez-Pastor, Barbaraen_US
dc.contributor.authorCuadrado, Myriamen_US
dc.contributor.authorYang, Seung-Minen_US
dc.contributor.authorBlasco, Maria A.en_US
dc.contributor.authorSkoultchi, Arthur I.en_US
dc.contributor.authorFernandez-Capetillo, Oscaren_US
dc.date.accessioned2009-12-08T17:51:02Z
dc.date.available2009-12-08T17:51:02Z
dc.date.issued2007-09-24
dc.identifier.citationMatilde Murga, Isabel Jaco, Yuhong Fan, Rebeca Soria, Barbara Martinez-Pastor, Myriam Cuadrado, Seung-Min Yang, Maria A. Blasco, Arthur I. Skoultchi, and Oscar Fernandez-Capetillo, “Global chromatin compaction limits the strength of the DNA damage response,” Journal of Cell Biology, (2007) Vol. 178, No. 7, 1101-1108.en_US
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/1853/31317
dc.description©2007 Murga et al.en_US
dc.descriptionDOI: 10.1083/jcb.200704140en_US
dc.description.abstractIn response to DNA damage, chromatin undergoes a global decondensation process that has been proposed to facilitate genome surveillance. However, the impact that chromatin compaction has on the DNA damage response (DDR) has not directly been tested and thus remains speculative. We apply two independent approaches (one based on murine embryonic stem cells with reduced amounts of the linker histone H1 and the second making use of histone deacetylase inhibitors) to show that the strength of the DDR is amplified in the context of “open” chromatin. H1-depleted cells are hyperresistant to DNA damage and present hypersensitive checkpoints, phenotypes that we show are explained by an increase in the amount of signaling generated at each DNA break. Furthermore, the decrease in H1 leads to a general increase in telomere length, an as of yet unrecognized role for H1 in the regulation of chromosome structure. We propose that slight differences in the epigenetic confi guration might account for the cell-to-cell variation in the strength of the DDR observed when groups of cells are challenged with DNA breaks.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectDNA damage responseen_US
dc.subjectChromatin compactionen_US
dc.subjectTelomere lengthen_US
dc.subjectChromatinen_US
dc.titleGlobal chromatin compaction limits the strength of the DNA damage responseen_US
dc.typeArticleen_US
dc.contributor.corporatenameSpanish National Cancer Center. Molecular Oncology Programmeen_US
dc.contributor.corporatenameAlbert Einstein College of Medicine. Dept. of Cell Biologyen_US
dc.publisher.originalRockefeller University Pressen_US
dc.identifier.doi10.1083/jcb.200704140


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