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dc.contributor.authorGassie, Donald V.en_US
dc.contributor.authorLenz, Petra H.en_US
dc.contributor.authorYen, Jeannetteen_US
dc.contributor.authorHartline, Daniel K.en_US
dc.date.accessioned2010-01-22T18:48:47Z
dc.date.available2010-01-22T18:48:47Z
dc.date.issued1993-07
dc.identifier.citationGassie, Donald V.; Lenz, Petra H.; Yen, Jeannette; Hartline, Daniel K., "Mechanoreception in Zooplankton First Antennae: Electrophysiological Techniques," Bulletin of Marine Science, 53(1): 96-105,1993.en
dc.identifier.issn0007-4977
dc.identifier.urihttp://hdl.handle.net/1853/31490
dc.description.abstractWe describe methods for delivering calibrated mechanical displacements to antennal mechanoreceptors in zooplankton while simultaneously recording related neural traffic. Mechanosensory neural responses to small water displacements (0.0 1-1 μm) were studied over a wide frequency range (30 to >3000 Hz) Receptors could be localized and properties (thresholds, phase locking, habituation) examined. These methods have been tested on calanoid copepod first antennae (antennules), but may be suitable for other preparations. Extracellular recordings are made by holding the animal in stainless steel forceps and raising it into a layer of mineral oil, leaving one of the first antennae projecting into the underlying sea water. Nerve impulse traffic is recorded between the forceps and a chlorided silver wire in the seawater. Antennae are stimulated by water displacements produced by a vibrating sphere attached to either an electromagnetic or piezoekctric transducer. A fiberoptic sensor continuously monitors displacement. A computer-controlled waveform generator and amplifier drive the transducer with various frequencies, amplitudes and waveforms. The amplified sensor output and neural activity are digitized for later analysisen
dc.language.isoen_USen
dc.publisherGeorgia Institute of Technologyen
dc.subjectMechanoreceptorsen
dc.subjectZooplanktonen
dc.subjectMechanosensory neural responsesen
dc.subjectWater displacementsen
dc.subjectCopepodsen
dc.subjectAntennaeen
dc.subjectAntennules
dc.titleMechanoreception in Zooplankton First Antennae: Electrophysiological Techniquesen
dc.typeArticleen
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.publisher.originalUniversity of Miami - Rosenstiel School of Marine and Atmospheric Science


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