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dc.contributor.authorBowen, Nathan J.en_US
dc.contributor.authorWalker, L. DeEtteen_US
dc.contributor.authorMatyunina, Lilya V.en_US
dc.contributor.authorLogani, Sanjayen_US
dc.contributor.authorTotten, Kimberly A.en_US
dc.contributor.authorBenigno, Benedict B.en_US
dc.contributor.authorMcDonald, John F.en_US
dc.date.accessioned2010-04-05T16:30:39Z
dc.date.available2010-04-05T16:30:39Z
dc.date.issued2009-12-29
dc.identifier.citationNathan J. Bowen, L. DeEtte Walker, Lilya V. Matyunina, Sanjay Logani, Kimberly A. Totten, Benedict B. Benigno, and John F. McDonald, "Gene expression profiling supports the hypothesis that human ovarian surface epithelia are multipotent and capable of serving as ovarian cancer initiating cells," BMC Medical Genomics 2009, 2:71en
dc.identifier.issn1755-8794
dc.identifier.urihttp://hdl.handle.net/1853/32511
dc.description© 2009 Bowen et al; licensee BioMed Central Ltd. The electronic version of this article is the complete one and can be found online at:http://www.biomedcentral.com/1755-8794/2/71en
dc.descriptionDOI: 10.1186/1755-8794-2-71
dc.description.abstractBackground Accumulating evidence suggests that somatic stem cells undergo mutagenic transformation into cancer initiating cells. The serous subtype of ovarian adenocarcinoma in humans has been hypothesized to arise from at least two possible classes of progenitor cells: the ovarian surface epithelia (OSE) and/or an as yet undefined class of progenitor cells residing in the distal end of the fallopian tube. Methods Comparative gene expression profiling analyses were carried out on OSE removed from the surface of normal human ovaries and ovarian cancer epithelial cells (CEPI) isolated by laser capture micro-dissection (LCM) from human serous papillary ovarian adenocarcinomas. The results of the gene expression analyses were randomly confirmed in paraffin embedded tissues from ovarian adenocarcinoma of serous subtype and non-neoplastic ovarian tissues using immunohistochemistry. Differentially expressed genes were analyzed using gene ontology, molecular pathway, and gene set enrichment analysis algorithms. Results Consistent with multipotent capacity, genes in pathways previously associated with adult stem cell maintenance are highly expressed in ovarian surface epithelia and are not expressed or expressed at very low levels in serous ovarian adenocarcinoma. Among the over 2000 genes that are significantly differentially expressed, a number of pathways and novel pathway interactions are identified that may contribute to ovarian adenocarcinoma development.en
dc.language.isoen_USen
dc.publisherGeorgia Institute of Technologyen
dc.subjectSomatic stem cellsen
dc.subjectMutagenic transformationen
dc.subjectOvarian surface epitheliaen
dc.subjectGene expression analysesen
dc.titleGene expression profiling supports the hypothesis that human ovarian surface epithelia are multipotent and capable of serving as ovarian cancer initiating cellsen
dc.typeArticleen
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Institute for Bioengineering and Bioscienceen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Ovarian Cancer Instituteen_US
dc.contributor.corporatenameEmory University. Dept. of Pathology and Laboratory Medicineen_US
dc.publisher.originalBioMed Central
dc.identifier.doi10.1186/1755-8794-2-71


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