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dc.contributor.authorMenendez, Lauraen_US
dc.contributor.authorWalker, L. DeEtteen_US
dc.contributor.authorMatyunina, Lilya V.en_US
dc.contributor.authorDickerson, Erin B.en_US
dc.contributor.authorBowen, Nathan J.en_US
dc.contributor.authorBenigno, Benedict B.en_US
dc.contributor.authorMcDonald, John F.en_US
dc.date.accessioned2010-04-05T17:37:21Z
dc.date.available2010-04-05T17:37:21Z
dc.date.issued2007-01-25
dc.identifier.citationLaura Menendez, L. DeEtte Walker, Lilya V. Matyunina, Erin B. Dickerson, Nathan J. Bowen, Nalini Polavarapu, Benedict B. Benigno and John F. McDonald, "Identification of candidate methylation-responsive genes in ovarian cancer," Molecular Cancer 2007, 6:10en
dc.identifier.issn1476-4598
dc.identifier.urihttp://hdl.handle.net/1853/32513
dc.description© 2007 Menendez et al; licensee BioMed Central Ltd. The electronic version of this article is the complete one and can be found online at: http://www.molecular-cancer.com/content/6/1/10en
dc.descriptionDOI: 10.1186/1476-4598-6-10
dc.description.abstractBackground: Aberrant methylation of gene promoter regions has been linked to changes in gene expression in cancer development and progression. Genes associated with CpG islands (CGIs) are especially prone to methylation, but not all CGI-associated genes display changes in methylation patterns in cancers. Results: In order to identify genes subject to regulation by methylation, we conducted gene expression profile analyses of an ovarian cancer cell line (OVCAR-3) before and after treatment with the demethylating agent 5-aza-deoxycytidine (5-aza-dC). An overlapping subset of these genes was found to display significant differences in gene expression between normal ovarian surface epithelial cells and malignant cells isolated from ovarian carcinomas. While 40% of all human genes are associated with CGIs, > 94% of the overlapping subset of genes is associated with CGIs. The predicted change in methylation status of genes randomly selected from the overlapping subset was experimentally verified. Conclusion: We conclude that correlating genes that are upregulated in response to 5-aza-dC treatment of cancer cell lines with genes that are down-regulated in cancer cells may be a useful method to identify genes experiencing epigenetic-mediated changes in expression over cancer development.en
dc.language.isoen_USen
dc.publisherGeorgia Institute of Technologyen
dc.subjectGene expression profilingen
dc.subjectOvarian tumorsen
dc.subjectEpigenetic-mediated changes in expressionen
dc.subjectMethylation-responsive genesen
dc.subjectDNA methylation
dc.titleIdentification of candidate methylation-responsive genes in ovarian canceren
dc.typeArticleen
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Ovarian Cancer Instituteen_US
dc.contributor.corporatenameUniversity of Georgia. Dept. of Geneticsen_US
dc.publisher.originalBioMed Central
dc.identifier.doi10.1186/1476-4598-6-10


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