A mannose receptor-like molecule likely serves as the mate recognition pheromone receptor in the male rotifer Brachionus manjavacas

Show full item record

Please use this identifier to cite or link to this item: http://hdl.handle.net/1853/33480

Title: A mannose receptor-like molecule likely serves as the mate recognition pheromone receptor in the male rotifer Brachionus manjavacas
Author: Couser, Laura M.
Abstract: Mate recognition in the male rotifer Brachionus manjavacas is controlled by contact chemoreception, which occurs when the male has a head-on encounter with a conspecific female. The female expresses a glycoprotein, the mate recognition pheromone or MRP, on her body surface that males detect with a receptor located in the male corona. A positive match between signal and receptor causes males to initiate mating behavior. After surveying the female B. manjavacas and male Brachionus plicatilis transcriptomes for all receptor genes, a C-type lectin gene was found which codes for a mannose receptor (MR). This gene is a good candidate for the MRP receptor because the MRP is glycosylated with mannose and mannose receptors recognize mannose/glucose oligosaccharides. Mannose has been found to block the binding of MR antibodies in males. In addition, mannose receptor immunostains show localized binding at the corona of males, but not in females. Mannose has also been shown to block circling, but binding of antibodies for the human mannose receptor does not decrease circling. A Western blot analysis of female rotifers shows that there is an MR in the female proteome; however, Western blots have not demonstrated binding of MR antibodies to male proteins. Mating bioassays showed that mannose is an important inhibitor of male mate recognition. These findings are best explained by the hypothesis that the male MRP receptor is an MR-like molecule that functions as a C-type lectin that is capable of binding of mannose.
Type: Undergraduate Thesis
URI: http://hdl.handle.net/1853/33480
Date: 2010-05-12
Publisher: Georgia Institute of Technology
Subject: MRP
Mate recognition pheromone receptor
Brachionus manjavacas
Mannose
Mate recognition
Mate recognition pheromone
Department: Biology
Advisor: Committee Member/Second Reader: Shearer, Tonya; Faculty Mentor: Snell, Terry W.

All materials in SMARTech are protected under U.S. Copyright Law and all rights are reserved, unless otherwise specifically indicated on or in the materials.

Files in this item

Files Size Format View
couser_laura_m_201005_ro.pdf 2.695Mb PDF View/ Open

This item appears in the following Collection(s)

Show full item record