The effects of tensile loading and extracellular environmental cues on fibroblastic differntiation and extracellular matrix production by mesenchymal stem cells

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dc.contributor.author Doroski, Derek M. en_US
dc.date.accessioned 2011-07-06T16:42:02Z
dc.date.available 2011-07-06T16:42:02Z
dc.date.issued 2011-03-22 en_US
dc.identifier.uri http://hdl.handle.net/1853/39523
dc.description.abstract Ligament/tendon tissue engineering has the potential to provide therapies that overcome the limitations of incomplete natural healing responses and inadequate graft materials. While ligament/tendon fibroblasts are an obvious choice of cell type for these applications, difficulties associated with finding a suitable cell source have limited their utility. Mesenchymal stem cells/marrow stromal cells (MSCs) are seen as a viable alternative since they can be harvested through routine medical procedures and can be differentiated toward a ligament/tendon fibroblast lineage. Further study is needed to create an optimal biomaterial/biomechanical environment for ligament/tendon fibroblastic differentiation of MSCs. The overall goal of this dissertation was to improve the understanding of the role that biomechanical stimulation and the biomaterial environment play, both independently and combined, on human MSC (hMSC) differentiation toward a ligament/tendon fibroblast phenotype. Specifically, the effects of cyclic tensile stimuli were studied in a biomaterial environment that provided controlled presentation of biological moieties. The influence of an enzymatically-degradable biomaterial environment on hMSC differentiation was investigated by creating biomaterials containing enzymatically-cleavable moieties. The role that preculture may play in tensile responses of hMSCs was also explored. Together, these studies provided insights into the contributions of the biomaterial and biomechanical environment to hMSC differentiation toward a ligament/tendon fibroblast phenotype. en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject GGGLGPAGGK en_US
dc.subject Matrix metalloproteinase en_US
dc.subject Oligo(poly(ethylene glycol) fumarate) en_US
dc.subject Hydrogel en_US
dc.subject.lcsh Mesenchymal stem cells Differentiation
dc.subject.lcsh Ligament prostheses
dc.subject.lcsh Tendons
dc.subject.lcsh Tissue engineering
dc.subject.lcsh Biomechanics
dc.title The effects of tensile loading and extracellular environmental cues on fibroblastic differntiation and extracellular matrix production by mesenchymal stem cells en_US
dc.type Dissertation en_US
dc.description.degree Ph.D. en_US
dc.contributor.department Biomedical Engineering en_US
dc.description.advisor Committee Chair: Temenoff, Johnna; Committee Member: Boyan, Barbara; Committee Member: Garcia, Andres; Committee Member: Levenston, Marc; Committee Member: Lyon, Andrew en_US


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