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    Engineering the pregnane X receptor and estrogen receptor alpha to bind novel small molecules using negative chemical complementation

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    shaffer_hally_a_201105_phd.pdf (13.96Mb)
    Date
    2011-04-05
    Author
    Shaffer, Hally A.
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    Abstract
    Nuclear receptors are ligand-activated transcription factors that play significant roles in various biological processes within the body, such as cell development, hormone metabolism, reproduction, and cardiac function. As transcription factors, nuclear receptors are involved in many diseases, such as diabetes, cancer, and arthritis, resulting in approximately 10-15% of the pharmaceutical drugs presently on the market being targeted toward nuclear receptors. Structurally, nuclear receptors consist of a DNA-binding domain (DBD), responsible for binding specific sequences of DNA called response elements, fused to a ligand-binding domain (LBD) through a hinge region. The LBD binds a small molecule ligand. Upon ligand binding, the LBD changes to an active conformation leading to the recruitment of coactivator (CoAC) proteins and initiation of transcription. As a result of their involvement in disease, there is an emphasis on engineering nuclear receptors for applications in gene therapy, drug discovery and metabolic engineering.
    URI
    http://hdl.handle.net/1853/39620
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    • Georgia Tech Theses and Dissertations [23878]
    • School of Chemistry and Biochemistry Theses and Dissertations [1525]

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