“What’s Eating You?” Quantifying Proteolytic Activity in Health and Disease with Novel Assays and Computational Models

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dc.contributor.author Platt, Manu O.
dc.date.accessioned 2011-09-26T15:57:11Z
dc.date.available 2011-09-26T15:57:11Z
dc.date.issued 2011-09-13
dc.identifier.uri http://hdl.handle.net/1853/41234
dc.description Presented on September 13, 2011 from 8:30-9:30 a.m. in the IBB Building room 1128 on the Georgia Tech campus. en_US
dc.description Runtime: 52:51 minutes
dc.description.abstract Cathepsins are enzymes with the most powerful human collagenase and elastase activity that are upregulated at sites of normal tissue remodeling and during tissue-destructive disease progression. We study them in the context of tissue remodeling in cancer progression and cardiovascular diseases such as sickle cell disease and atherosclerosis. They are synthesized as stable inactive precursors requiring activation by propeptide cleavage, and detection of mature cathepsins and quantification of specific activity have proven difficult due to instability of the mature, active enzyme extracellularly, diminishing appreciation for their involvement in a large number of diseases. During this seminar, we will discuss our studies of this family of powerful proteases in diseases with particular attention to cancer and sickle cell disease. First, we will discuss the important development of a reliable, sensitive method of zymography to detect the activity of mature cathepsins K, L, S, and V and integrating that assay with the development of a computational kinetic model to predict cathepsin-mediated tissue remodeling by cells during advancing disease. Secondly, we will discuss our applications of these technologies and potential use as both diagnostic and prognostic indicators of human breast, lung, and cervical cancer as well as newly identified mechanisms of cathepsin activity in complications of stroke in children with sickle cell disease. en_US
dc.format.extent 52:51 minutes
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.subject Cancer en_US
dc.subject Detection en_US
dc.subject Enzymes en_US
dc.subject HIV en_US
dc.subject Sickle cell en_US
dc.title “What’s Eating You?” Quantifying Proteolytic Activity in Health and Disease with Novel Assays and Computational Models en_US
dc.type Lecture en_US
dc.type Video en_US
dc.contributor.corporatename Georgia Institute of Technology. Institute for Bioengineering and Bioscience
dc.contributor.corporatename Georgia Institute of Technology. Dept. of Biomedical Engineering


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