In vitro Biomimetic Construction of Hydroxyapatite–Porcine Acellular Dermal Matrix Composite Scaffold for MC3T3-E1 Preosteoblast Culture

Abstract

The application of porous hydroxyapatite–collagen (HAp-Collagen) as a bone tissue engineering scaffold is hindered by two main problems: its high cost and low initial strength. As a native 3-dimenssional collagen framework, purified porcine acellular dermal matrix (PADM) has been successfully used as a skin tissue engineering scaffold. Here we report its application as a matrix for the preparation of HAp to produce a bone tissue scaffold through a biomimetic chemical process. The HAp-PADMscaffold has two-level pore structure, with large channels (*100 mm in diameter) inherited from the purified PADM microstructure and small pores (<100 nm in diameter) formed by self-assembled HAp on the channel surfaces. The obtained HAp-PADM scaffold (S15D) has a compressive elastic modulus as high as 600 kPa. The presence of HAp in sample S15D reduces the degradation rate of PADM in collagenase solution at 378C. After 7 day culture of MC3T3-E1 pre-osteroblasts, MTT data show no statistically significant difference on pure PADM framework and HAp-PADM scaffold ( p>0.05). Because of its high strength and nontoxicity, its simple preparation method, and designable and tailorable properties, the HAp- PADM scaffold is expected to have great potential applications in medical treatment of bone defects.