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dc.contributor.authorStewart, Frank J.en_US
dc.contributor.authorDmytrenko, Olegen_US
dc.contributor.authorDeLong, Edward F.en_US
dc.contributor.authorCavanaugh, Colleen M.en_US
dc.date.accessioned2012-01-24T14:09:06Z
dc.date.available2012-01-24T14:09:06Z
dc.date.issued2011-06-20
dc.identifier.citationStewart FJ, Dmytrenko O, DeLong EF, Cavanaugh CM. 2011. Metatranscriptomic analysis of sulfur oxidation genes in the endosymbiont of Solemya velum. Front. Microbiol. 2:134.en_US
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/1853/42272
dc.descriptionThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.en_US
dc.descriptionDOI: 10.3389/fmicb.2011.00134
dc.description.abstractThioautotrophic endosymbionts in the Domain Bacteria mediate key sulfur transformations in marine reducing environments. However, the molecular pathways underlying symbiont metabolism and the extent to which these pathways are expressed in situ are poorly characterized for almost all symbioses. This is largely due to the difficulty of culturing symbionts apart from their hosts. Here, we use pyrosequencing of community RNA transcripts (i.e., the metatranscriptome) to characterize enzymes of dissimilatory sulfur metabolism in the model symbiosis between the coastal bivalve Solemya velum and its intracellular thioautotrophic symbionts. High-throughput sequencing of total RNA from the symbiont-containing gill of a single host individual generated 1.6 million sequence reads (500 Mbp). Of these, 43,735 matched Bacteria protein-coding genes in BLASTX searches of the NCBI database. The taxonomic identities of the matched genes indicated relatedness to diverse species of sulfur-oxidizing Gammaproteobacteria, including other thioautotrophic symbionts and the purple sulfur bacterium Allochromatium vinosum. Manual querying of these data identified 28 genes from diverse pathways of sulfur energy metabolism, including the dissimilatory sulfite reductase (Dsr) pathway for sulfur oxidation to sulfite, the APS pathway for sulfite oxidation, and the Sox pathway for thiosulfate oxidation. In total, reads matching sulfur energy metabolism genes represented 7% of the Bacteria mRNA pool. Together, these data highlight the dominance of thioautotrophy in the context of symbiont community metabolism, identify the likely pathways mediating sulfur oxidation, and illustrate the utility of metatranscriptome sequencing for characterizing community gene transcription of uncultured symbionts.en_US
dc.language.isoen_USen
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectChemosynthesisen_US
dc.subjectEndosymbiosisen_US
dc.subjectSulfideen_US
dc.subjectThiosulfateen_US
dc.subjectGene expressionen_US
dc.titleMetatranscriptomic analysis of sulfur oxidation genes in the endosymbiont of Solemya velumen_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.contributor.corporatenameHarvard University. Dept. of Organismic and Evolutionary Biologyen_US
dc.contributor.corporatenameMassachusetts Institute of Technology. Dept. of Civil and Environmental Engineeringen_US
dc.publisher.originalFrontiers Research Foundationen_US
dc.identifier.doi10.3389/fmicb.2011.00134


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