On cyclodextrin inclusion complexes as shift reagents in traveling-wave ion mobility spectrometry
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Detection of lipids and lipid/cyclodextrin complexes is a nontrivial aspect in novel treatments of Niemann-Pick disease type C. Recently, the FDA approved Hydroxypropyl-β-cyclodextrin as a treatment for the condition, as it had shown promising results in mice in the removal of neurodegeneration biomarkers.1 Several lipids of concern in Niemann-Pick disease type C, and their corresponding cyclodextrin complexes were detected via traveling-wave ion mobility mass spectrometry (T-Wave-MS). In addition to detection alone by MS, collision cross sections (CCS) of the ions were measured within the T-Wave cell, to give insight as to the size of the complexes. As shown, using cyclodextrins to complex lipids shifts their place within the m/z vs. CCS space, aiding the spectral interpretation process for detecting the complexes. Additionally, a study of how wave height within the T-Wave cell effects the CCSs obtained was performed. Here, evidence for dipole alignment within the T-Wave cell is presented, as ions of greater rigidity show smaller CCSs at greater wave heights.