Ampicillin Resistance in Fecal Coliforms of Canoochee River
Gleason, Michael L.
McGill, Kenneth C.
Owen, Lee P.
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In Spring 2000, the Canoochee River was sampled at eight sites from N32°22.34', W82°7.42' to N32° 10.74, W81°49.66' and the samples quantitated by EPA methods for fecal coliform and fecal Streptococci. Fecal coliform levels ranged from 140-540 fecal coliforms/100 ml and coliform/streptococcal ratios ranged from 3.2-10. Fecal coliforms were also tested for resistance to the antibiotic ampicillin (frequency of resistance = 0.11±0.036). Plasmid DNA isolated from selected fecal coliforms conferred resistance to Escherichia coli K12-JM101 upon transformation.