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dc.contributor.authorHill, Jennifer L.
dc.contributor.authorHartel, Peter G.
dc.contributor.authorSegars, William I.
dc.contributor.authorBush, Parshall B.
dc.contributor.editorHatcher, Kathryn J.
dc.date.accessioned2012-07-20T17:15:51Z
dc.date.available2012-07-20T17:15:51Z
dc.date.issued2001-03
dc.identifier.isbn0-935835-07-5
dc.identifier.urihttp://hdl.handle.net/1853/44373
dc.descriptionProceedings of the 2001 Georgia Water Resources Conference, April 26 and 27, 2001, Athens, Georgia.en_US
dc.description.abstractThe Bleckley County Health Department reported that three households near Cochran, Georgia tested positive for fecal coliforms in their wells. Fecal coliforms are bacteria that are a measure of fecal contamination and are typically found in the intestines of warm-blooded animals, including humans. We were asked to isolate one bacterial species of these fecal coliforrns, Escherichia coli, and to determine the source of the isolates by ribotyping. Ribotyping is a method to identify a subspecies of a bacterium by comparing differences in their DNA. We quantified the number of E. coli in water samples from one pond, two streams, a sinkhole, and three household wells and their accompanying septic systems. The pond, streams, and sinkhole are all connected. We ribotyped 51 E. coli isolates. Twelve different ribotypes were observed among the water sources and the household wells with their accompanying septic systems. Two ribotype patterns were observed from the septic systems, ten patterns among the pond, sinkhole, and two streams, and six patterns among the three household wells. At 100% similarity, all the ribotype patterns of E. coli from the household wells were associated with patterns from the pond, sinkhole, or two streams. The similarity of E. coli ribotype patterns from the household wells with the septic systems were only 80 and 86%. The results suggest that the point source of the E. coli contamination was the pond, sinkhole, or two streams, and not the septic systems.en_US
dc.description.sponsorshipSponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, Natural Resources Conservation Service, The University of Georgia, Georgia State University, Georgia Institute of Technologyen_US
dc.description.statementofresponsibilityThis book was published by the Institute of Ecology, The University of Georgia, Athens, Georgia 30602-2202. The views and statements advanced in this publication are solely those of the authors and do not represent official views or policies of The University of Georgia, the U.S. Geological Survey, the Georgia Water Research Institute as authorized by the Water Resources Research Act of 1990 (P.L. 101-397) or the other conference sponsors.
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.relation.ispartofseriesGWRI2001. Groundwater and Coastal Issuesen_US
dc.subjectWater resources managementen_US
dc.subjectBacteriaen_US
dc.subjectContaminationen_US
dc.subjectWater qualityen_US
dc.subjectWater supplyen_US
dc.titleRibotyping to Determine the Source of Fecal Coliform Contamination in Three Household Wells Near Cochran, Georgiaen_US
dc.typeProceedingsen_US
dc.contributor.corporatenameUniversity of Georgia. Dept. of Crop and Soil Sciences
dc.contributor.corporatenameUniversity of Georgia. Agricultural and Environmental Services Laboratories
dc.publisher.originalInstitute of Ecology


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