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dc.contributor.authorPak, Nikitaen_US
dc.date.accessioned2012-09-20T18:24:09Z
dc.date.available2012-09-20T18:24:09Z
dc.date.issued2012-07-16en_US
dc.identifier.urihttp://hdl.handle.net/1853/44901
dc.description.abstractThe polymerase chain reaction (PCR) is an extremely powerful tool for viral detection and screening because it can detect specific infectious agents with great sensitivity and specificity. It works by exponentially amplifying a target viral DNA sequence to high enough concentrations through the use of specific reagents and thermal cycling. It has surpassed culture based methods as the gold standard for viral detection because of the increased speed and sensitivity. Microfluidic approaches to PCR have focused on decreasing the time to thermally cycle, the volumes used for reactions, and they have also added upstream and downstream processes that are of benefit for on-chip viral detection. While these improvements have made great strides over commercially available products in terms of speed, cost, and integration, a major limitation that has yet to be explored is the throughput associated with running PCR. Since each PCR reaction relies on primers with a unique annealing temperature to detect specific viral DNA, only a single virus can be screened for at a time. The device presented here uses two infrared laser diodes that are driven identically by the same laser driver to independently thermally cycle two chambers on the same microfluidic chip. Different temperatures are achieved in the two chambers by modulating the radiation reaching one of those chambers with an optical shutter. Closed loop temperature feedback in both chambers is done with a Labview program and thermocouples embedded in the polymer chip. This allows for accurate temperature measurement without inhibiting the reaction. To demonstrate the capabilities of this device, two different reactions were simultaneously amplified successfully on the same device that have annealing temperatures that differ by 15°C.en_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectPCRen_US
dc.subjectVirus detectionen_US
dc.subjectInfrareden_US
dc.subjectHigh-throughputen_US
dc.subjectAnnealingen_US
dc.subject.lcshPolymerase chain reaction
dc.subject.lcshGene amplification
dc.subject.lcshMicrofluidic devices
dc.titleSimultaneous amplification of multiple dna targets with optimized annealing temperaturesen_US
dc.typeThesisen_US
dc.description.degreeMSen_US
dc.contributor.departmentMechanical Engineeringen_US
dc.description.advisorCommittee Chair: Forest, Craig; Committee Member: Cola, Baratunde; Committee Member: Sulchek, Todden_US


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