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    Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles

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    Date
    2011-03
    Author
    Maurin, Mathieu
    Stéphan, Olivier
    Vial, Jean-Claude
    Marder, Seth R.
    Van der Sanden, Boudewijn
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    Abstract
    The purpose of this work was to validate the use of Pluronic fluorescent nanomicelles for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vascular endothelium was compared to a classic blood pool dye Rhodamin B dextran (70 kDa) using two-photon microscopy. Pluronic nanomicelles showed, like Rhodamin B dextran, a homogeneous blood plasma staining for at least 1 hour after intravenous injection. Their two-photon imaging depth was similar in normal mouse brain using 10 times less injected mass. In contrast with Rhodamin B dextran, no extravasation is observed in leaky tumor vessels due to their large size: 20-100 nm. In conclusion, Pluronic nanomicelles can be used as a blood pool dye, even in leaky tumor vessels. The use of Pluronic block co-polymers is a valuable approach for encapsulating twophoton fluorescent dyes that are hydrophobic and not suitable for intravenous injection.
    URI
    http://hdl.handle.net/1853/45276
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