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dc.contributor.authorMaurin, Mathieuen_US
dc.contributor.authorStéphan, Olivieren_US
dc.contributor.authorVial, Jean-Claudeen_US
dc.contributor.authorMarder, Seth R.en_US
dc.contributor.authorVan der Sanden, Boudewijnen_US
dc.date.accessioned2012-11-08T21:22:48Z
dc.date.available2012-11-08T21:22:48Z
dc.date.issued2011-03
dc.identifier.citationMaurin, Mathieu and Stephan, Olivier and Vial, Jean-Claude and Marder, Seth R. and van der Sanden, Boudewijn, "Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles," Journal of Biomedical Optics, 16, 3, (March 2011)en_US
dc.identifier.issn1083-3668
dc.identifier.urihttp://hdl.handle.net/1853/45276
dc.description© 2011 SPIE--The International Society for Optical Engineering. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. The electronic version of this article is the complete one and can be found online at: http://dx.doi.org/10.1117/1.3548879en_US
dc.descriptionDOI: 10.1117/1.3548879en_US
dc.description.abstractThe purpose of this work was to validate the use of Pluronic fluorescent nanomicelles for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vascular endothelium was compared to a classic blood pool dye Rhodamin B dextran (70 kDa) using two-photon microscopy. Pluronic nanomicelles showed, like Rhodamin B dextran, a homogeneous blood plasma staining for at least 1 hour after intravenous injection. Their two-photon imaging depth was similar in normal mouse brain using 10 times less injected mass. In contrast with Rhodamin B dextran, no extravasation is observed in leaky tumor vessels due to their large size: 20-100 nm. In conclusion, Pluronic nanomicelles can be used as a blood pool dye, even in leaky tumor vessels. The use of Pluronic block co-polymers is a valuable approach for encapsulating twophoton fluorescent dyes that are hydrophobic and not suitable for intravenous injection.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectIntravital two-photon microscopyen_US
dc.subjectCerebral and tumor microvasculatureen_US
dc.subjectPluronic nanomicellesen_US
dc.titleDeep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicellesen_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Center for Organic Photonics and Electronicsen_US
dc.contributor.corporatenameUniversité scientifique et médicale de Grenoble. Laboratoire Interdisciplinaire de Physiqueen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Chemistry and Biochemistryen_US
dc.contributor.corporatenameCentre national de la recherche scientifique (France)en_US
dc.contributor.corporatenameGrenoble Institut des Neurosciencesen_US
dc.contributor.corporatenameInstitut national de la santé et de la recherche médicale (France)en_US
dc.publisher.originalSociety of Photo-Optical Instrumentation Engineersen_US
dc.identifier.doi10.1117/1.3548879


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