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dc.contributor.authorKasson, Tina Michelle Dreadenen_US
dc.date.accessioned2013-01-17T20:47:38Z
dc.date.available2013-01-17T20:47:38Z
dc.date.issued2012-08-08en_US
dc.identifier.urihttp://hdl.handle.net/1853/45759
dc.description.abstractOxidative stress is a natural consequence of photosynthetic oxygen evolution and redox enzyme processes. Trp oxidation to N-formylkynurenine (NFK) is a specific, reactive oxygen species (ROS)-mediated reaction. This thesis work describes the identification and functional characterization of NFK in oxygen evolving Photosystem II (PSII). Although proteomics studies have confirmed NFK modifications in many types of proteins, limited knowledge on the biochemical significance exists. In vitro studies in thylakoids and PSII membranes were used to establish a correlation between oxidative stress, NFK formation, and photoinhibition. The in vivo effect of preventing Trp oxidation to NFK was assessed by site-directed mutation in the cyanobacteria Synechocystis sp. PCC 6803. This work provides insight into the role of NFK in photosynthetic oxygen evolution and photoinhibition. Based on the current knowledge of NFK, ROS, and repair, a new model is described. In this modified model for photoinhibition and repair, NFK plays a role in signaling for turnover of damaged proteins. NFK may play a similar role in replacement of damaged proteins in other systems.en_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectTryptophanen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectPhotosynthesisen_US
dc.subjectPhotosystem IIen_US
dc.subjectN-formylkynurenineen_US
dc.subjectSynechocystis 6803en_US
dc.subject.lcshAmino acids
dc.subject.lcshPlants Effect of light on
dc.titleHigh light stress in photosynthesis: the role of oxidative post-translational modifications in signaling and repairen_US
dc.typeDissertationen_US
dc.description.degreePhDen_US
dc.contributor.departmentChemistry and Biochemistryen_US
dc.description.advisorCommittee Chair: Bridgette A. Barry; Committee Member: David Collard; Committee Member: Ingeborg Schmidt-Krey; Committee Member: Wendy Kelly; Committee Member: Yomi Oyelereen_US


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