Show simple item record

dc.contributor.authorYago, Tadayukien_US
dc.contributor.authorWu, Jianhuaen_US
dc.contributor.authorWey, C. Dianaen_US
dc.contributor.authorKlopocki, Arkadiusz G.en_US
dc.contributor.authorZhu, Chengen_US
dc.contributor.authorMcEver, Rodger P.en_US
dc.date.accessioned2013-06-03T20:16:36Z
dc.date.available2013-06-03T20:16:36Z
dc.date.issued2004-09
dc.identifier.citationTadayuki Yago, Jianhua Wu, C. Diana Wey, Arkadiusz G. Klopocki, Cheng Zhu, and Rodger P. McEver, "Catch bonds govern adhesion through L-selectin at threshold shear," Journal of cell biology, 166, 6, 913-923 (September 13, 2004)en_US
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/1853/47161
dc.description© 2004 The Authorsen_US
dc.descriptionThe electronic version of this article is the complete one and can be found online at: http://www.jcb.org/cgi/doi/10.1083/jcb.200403144en_US
dc.descriptionDOI: 10.1083/jcb.200403144en_US
dc.description.abstractFlow-enhanced cell adhesion is an unexplained phenomenon that might result from a transport-dependent increase in on-rates or a force-dependent decrease in off-rates of adhesive bonds. L-selectin requires a threshold shear to support leukocyte rolling on P-selectin glycoprotein ligand-1 (PSGL-1) and other vascular ligands. Low forces decrease L-selectin–PSGL-1 off-rates (catch bonds), whereas higher forces increase off-rates (slip bonds). We determined that a force-dependent decrease in off-rates dictated flowenhanced rolling of L-selectin–bearing microspheres or neutrophils on PSGL-1. Catch bonds enabled increasing force to convert short-lived tethers into longer-lived tethers, which decreased rolling velocities and increased the regularity of rolling steps as shear rose from the threshold to an optimal value. As shear increased above the optimum, transitions to slip bonds shortened tether lifetimes, which increased rolling velocities and decreased rolling regularity. Thus, force-dependent alterations of bond lifetimes govern L-selectin–dependent cell adhesion below and above the shear optimum. These findings establish the first biological function for catch bonds as a mechanism for flow-enhanced cell adhesion.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectCatch bondsen_US
dc.subjectFlow-enhanced cell adhesionen_US
dc.subjectL-selectinen_US
dc.subjectP-selectinen_US
dc.subjectLigandsen_US
dc.subjectShear thresholden_US
dc.subjectAdhesionen_US
dc.titleCatch bonds govern adhesion through L-selectin at threshold shearen_US
dc.typeArticleen_US
dc.contributor.corporatenameOklahoma Medical Research Foundation. Cardiovascular Biology Research Programen_US
dc.contributor.corporatenameUniversity of Oklahoma Health Sciences Center. Dept. of Biochemistry and Molecular Biologyen_US
dc.contributor.corporatenameUniversity of Oklahoma Health Sciences Center. Oklahoma Center for Medical Glycobiologyen_US
dc.contributor.corporatenameZhongshan University. School of Life Sciencesen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Dept. of Biomedical Engineeringen_US
dc.contributor.corporatenameEmory University. Dept. of Biomedical Engineeringen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Mechanical Engineeringen_US
dc.publisher.originalRockefeller University Pressen_US
dc.identifier.doi10.1083/jcb.200403144


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record