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dc.contributor.authorSantangelo, Philip J.en_US
dc.contributor.authorNix, Brenten_US
dc.contributor.authorTsourkas, Andrewen_US
dc.contributor.authorBao, Gangen_US
dc.date.accessioned2013-06-03T20:16:36Z
dc.date.available2013-06-03T20:16:36Z
dc.date.issued2004
dc.identifier.citationPhilip J. Santangelo, Brent Nix, Andrew Tsourkas and Gang Bao, “Dual FRET molecular beacons for mRNA detection in living cells,” Nucleic Acids Research, 32, e57, 2004.en_US
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/1853/47167
dc.description© The Author(s) 2004. Published by Oxford University Press.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.descriptionDOI: 10.1093/nar/gnh062en_US
dc.description.abstractThe ability to visualize in real-time the expression level and localization of specific endogenous RNAs in living cells can offer tremendous opportunities for biological and disease studies. Here we demonstrate such a capability using a pair of molecular beacons, one with a donor and the other with an acceptor fluorophore that hybridize to adjacent regions on the same mRNA target, resulting in fluorescence resonance energy transfer (FRET). Detection of the FRET signal significantly reduced false positives, leading to sensitive imaging of K-ras and survivin mRNAs in live HDF and MIAPaCa-2 cells. FRET detection gave a ratio of 2.25 of K-ras mRNA expression in stimulated and unstimulated HDF, comparable to the ratio of 1.95 using RT±PCR, and in contrast to the single-beacon result of 1.2. We further revealed intriguing details of K-ras and survivin mRNA localization in living cells. The dual FRET molecular beacons approach provides a novel technique for sensitive RNA detection and quantification in living cells.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectReal-time visualizationen_US
dc.subjectRNAen_US
dc.subjectFluorescence resonance energy transferen_US
dc.subjectFRETen_US
dc.subjectMolecular beaconsen_US
dc.titleDual FRET molecular beacons for mRNA detection in living cellsen_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Dept. of Biomedical Engineeringen_US
dc.contributor.corporatenameEmory University. Dept. of Biomedical Engineeringen_US
dc.publisher.originalOxford University Pressen_US
dc.identifier.doi10.1093/nar/gnh062


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