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dc.contributor.authorKarathanasis, Efstathiosen_US
dc.contributor.authorChan, Leslieen_US
dc.contributor.authorKarumbaiah, Lohitashen_US
dc.contributor.authorMcNeeley, Kathleenen_US
dc.contributor.authorD’Orsi, Carl J.en_US
dc.contributor.authorAnnapragada, Ananth V.en_US
dc.contributor.authorSechopoulos, Ioannis S.en_US
dc.contributor.authorBellamkonda, Ravi Venkaten_US
dc.date.accessioned2013-06-13T17:29:37Z
dc.date.available2013-06-13T17:29:37Z
dc.date.issued2009
dc.identifier.citationEfstathios Karathanasis, Leslie Chan, Lohitash Karumbaiah, Kathleen McNeeley, Carl J. D’Orsi, Ananth V. Annapragada, Ioannis Sechopoulos, Ravi V. Bellamkonda, "Tumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markers," PLoS ONE 4,6, e5843 (2009)en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1853/47466
dc.description© 2009 Karathanasis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.descriptionDOI: 10.1371/journal.pone.0005843en_US
dc.description.abstractBackground. Vascular endothelial growth factor (VEGF) receptor-2 is the major mediator of the mitogenic, angiogenic, and vascular hyperpermeability effects of VEGF on breast tumors. Overexpression of VEGF and VEGF receptor-2 is associated with the degree of pathomorphosis of the tumor tissue and unfavorable prognosis. In this study, we demonstrate that non-invasive quantification of the degree of tumor vascular permeability to a nanoprobe correlates with the VEGF and its receptor levels and tumor growth. Methodology/Principal Findings. We designed an imaging nanoprobe and a methodology to detect the intratumoral deposition of a 100 nm-scale nanoprobe using mammography allowing measurement of the tumor vascular permeability in a rat MAT B III breast tumor model. The tumor vascular permeability varied widely among the animals. Notably, the VEGF and VEGF receptor-2 gene expression of the tumors as measured by qRT-PCR displayed a strong correlation to the imaging-based measurements of vascular permeability to the 100 nm-scale nanoprobe. This is in good agreement with the fact that tumors with high angiogenic activity are expected to have more permeable blood vessels resulting in high intratumoral deposition of a nanoscale agent. In addition, we show that higher intratumoral deposition of the nanoprobe as imaged with mammography correlated to a faster tumor growth rate. This data suggest that vascular permeability scales to the tumor growth and that tumor vascular permeability can be a measure of underlying VEGF and VEGF receptor-2 expression in individual tumors. Conclusions/Significance. This is the first demonstration, to our knowledge, that quantitative imaging of tumor vascular permeability to a nanoprobe represents a form of a surrogate, functional biomarker of underlying molecular markers of angiogenesisen_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectVascular endothelial growth factoren_US
dc.subjectVEGF receptor-2en_US
dc.subjectBreast tumorsen_US
dc.subjectImaging nanoprobeen_US
dc.subjectTumor vascular permeabilityen_US
dc.subjectBiomarkersen_US
dc.titleTumor vascular permeability to a nanoprobe correlates to tumor-specific expression levels of angiogenic markersen_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Dept. of Biomedical Engineeringen_US
dc.contributor.corporatenameEmory University. Dept. of Biomedical Engineeringen_US
dc.contributor.corporatenameWinship Cancer Institute of Emory Universityen_US
dc.contributor.corporatenameEmory University. School of Medicine. Dept. of Radiologyen_US
dc.contributor.corporatenameUniversity of Texas at Houston. School of Health Information Sciencesen_US
dc.publisher.originalPublic Library of Scienceen_US
dc.identifier.doi10.1371/journal.pone.0005843


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