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dc.contributor.authorCao, Kaixiangen_US
dc.contributor.authorLailler, Nathalieen_US
dc.contributor.authorZhang, Yunzheen_US
dc.contributor.authorKumar, Ashwathen_US
dc.contributor.authorUppal, Karanen_US
dc.contributor.authorLiu, Zhengen_US
dc.contributor.authorLee, Eva K.en_US
dc.contributor.authorWu, Hongweien_US
dc.contributor.authorMedrzycki, Magdalenaen_US
dc.contributor.authorPan, Chenyien_US
dc.contributor.authorHo, Po-Yien_US
dc.contributor.authorCooper, Guy P. , Jr.en_US
dc.contributor.authorDong, Xiaoen_US
dc.contributor.authorBock, Christophen_US
dc.contributor.authorBouhassira, Eric E.en_US
dc.contributor.authorFan, Yuhongen_US
dc.date.accessioned2013-09-27T20:22:46Z
dc.date.available2013-09-27T20:22:46Z
dc.date.issued2013-04
dc.identifier.citationCao K., Lailler N., Zhang Y., Kumar A., Uppal K., Liu Z., Lee E.K., Wu H., Medrzycki M., Pan C., Ho P.Y., Cooper G.P., Jr, Dong X., Bock C., Bouhassira E.E., Fan Y., "High-resolution mapping of h1 linker histone variants in embryonic stem cells," PLoS Genetics, 9(4):e1003417 (2013).en_US
dc.identifier.issn1553-7390
dc.identifier.urihttp://hdl.handle.net/1853/49156
dc.description© 2013 Cao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.descriptionDOI: 10.1371/journal.pgen.1003417.en_US
dc.description.abstractH1 linker histones facilitate higher-order chromatin folding and are essential for mammalian development. To achieve highresolution mapping of H1 variants H1d and H1c in embryonic stem cells (ESCs), we have established a knock-in system and shown that the N-terminally tagged H1 proteins are functionally interchangeable to their endogenous counterparts in vivo. H1d and H1c are depleted from GC- and gene-rich regions and active promoters, inversely correlated with H3K4me3, but positively correlated with H3K9me3 and associated with characteristic sequence features. Surprisingly, both H1d and H1c are significantly enriched at major satellites, which display increased nucleosome spacing compared with bulk chromatin. While also depleted at active promoters and enriched at major satellites, overexpressed H10 displays differential binding patterns in specific repetitive sequences compared with H1d and H1c. Depletion of H1c, H1d, and H1e causes pericentric chromocenter clustering and de-repression of major satellites. These results integrate the localization of an understudied type of chromatin proteins, namely the H1 variants, into the epigenome map of mouse ESCs, and we identify significant changes at pericentric heterochromatin upon depletion of this epigenetic mark.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectChromatinen_US
dc.subjectEmbryonic stem cellsen_US
dc.subjectEpigeneticsen_US
dc.subjectGene expressionen_US
dc.subjectHigh performance liquid chromatographyen_US
dc.subjectHistonesen_US
dc.subjectMammalian genomicsen_US
dc.subjectNucleosomesen_US
dc.titleHigh-resolution mapping of h1 linker histone variants in embryonic stem cells.en_US
dc.typeArticleen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.contributor.corporatenameGeorgia Institute of Technology. Institute for Bioengineering and Bioscienceen_US
dc.contributor.corporatenameAlbert Einstein College of Medicine. Dept. of Medicineen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Industrial and Systems Engineeringen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Electrical and Computer Engineeringen_US
dc.contributor.corporatenameResearch Center for Molecular Medicine of the Austrian Academy of Sciencesen_US
dc.contributor.corporatenameMedizinische Universität Wienen_US
dc.contributor.corporatenameMax-Planck-Institut für Informatiken_US
dc.publisher.originalPublic Library of Scienceen_US
dc.identifier.doi10.1371/journal.pgen.1003417.


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