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dc.contributor.authorBurck, Carol L.en_US
dc.contributor.authorChernoff, Yury O.en_US
dc.contributor.authorLiu, Rongen_US
dc.contributor.authorFarabaugh, Philip J.en_US
dc.contributor.authorLiebman, Susan W.en_US
dc.date.accessioned2013-10-07T18:55:26Z
dc.date.available2013-10-07T18:55:26Z
dc.date.issued1999-05
dc.identifier.citationC.L. Burck, Y.O. Chernoff, R.Liu, P.J. Farabaugh and S.W. Liebman, "Translational suppressors and antisuppressors alter the efficiency of the Ty1 programmed translational frameshift," RNA (May 1999) 5(11): 1451-1457.en_US
dc.identifier.issn1355-8382
dc.identifier.urihttp://hdl.handle.net/1853/49184
dc.description©1999 RNA Society. Published by Cold Spring Harbor Laboratoryen_US
dc.description.abstractCertain viruses, transposons, and cellular genes have evolved specific sequences that induce high levels of specific translational errors. Such “programmed misreading” can result in levels of frameshifting or nonsense codon readthrough that are up to 1,000-fold higher than normal. Here we determine how a number of mutations in yeast affect the programmed misreading used by the yeast Ty retrotransposons. These mutations have previously been shown to affect the general accuracy of translational termination. We find that among four nonsense suppressor ribosomal mutations tested, one (a ribosomal protein mutation) enhanced the efficiency of the Ty1 frameshifting, another (an rRNA mutation) reduced frameshifting, and two others (another ribosomal protein mutation and another rRNA mutation) had no effect. Three antisuppressor rRNA mutations all reduced Ty1 frameshifting; however the antisuppressor mutation in the ribosomal protein did not show any effect. Among nonribosomal mutations, the allosuppressor protein phosphatase mutation enhanced Ty1 frameshifting, whereas the partially inactive prion form of the release factor eRF3 caused a slight decrease, if any effect. A mutant form of the other release factor, eRF1, also had no effect on frameshifting. Our data suggest that Ty frameshifting is under the control of the cellular translational machinery. Surprisingly we find that translational suppressors can affect Ty frameshifting in either direction, whereas antisuppressors have either no effect or cause a decrease.en_US
dc.language.isoen_USen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectProgrammed misreadingen_US
dc.subjectrDNAen_US
dc.subjectRetrotransposonen_US
dc.subjectTranslational accuracyen_US
dc.subjectYeasten_US
dc.titleTranslational Suppressors and Antisuppressors Alter the Efficiency of the Ty1 Translational Frameshiften_US
dc.typeArticleen_US
dc.contributor.corporatenameUniversity of Illinois at Chicago. Dept. of Biological Sciencesen_US
dc.contributor.corporatenameUniversity of Maryland, Baltimore. Dept. of Biological Sciencesen_US
dc.contributor.corporatenameHarvard University. Dept. of Cancer Cell Biologyen_US
dc.contributor.corporatenameGeorgia Institute of Technology. School of Biologyen_US
dc.contributor.corporatenameAaron Diamond AIDS Research Centeren_US
dc.publisher.originalCold Spring Harbor Laboratory Pressen_US


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