Factors that Increase the Efficiency of DNA Repair when Using Transcript RNA as a Template
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Interaction with various chemicals and environmental agents can cause damages to DNA. In this study, we particularly focus on double stranded breaks (DSBs) and a repair mechanism that uses transcript RNA to repair the break. A previous study has shown that the frequency of DSB repair by transcript RNA in the cis system is increased in cells with a genotype of spt3 rnh1 rnh201. This research focuses on determining factors that increase the efficiency of DSB repair when using transcript RNA as a template. Cells with the genotype of spt3 rnh1 rnh201 in a cis-system were mutated and transformed with gene over expression library in two separate experiments. We have found 13 candidates from 20,000 mutated cells that can improve the efficiency of repair using transcript RNA, as well as 58 potential candidates out of 3,784 cells transformed with the yeast gene over expression library.