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    Electrosonic Cell Manipulation Device And Method Of Use Thereof

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    7704743.pdf (1.495Mb)
    Date
    4/27/2010
    Author
    Fedorov, Andrei G.
    Degertekin, Fahrettin L.
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    Abstract
    In method of injecting a substance into a living cell having a cell membrane, the substance, the cell and a liquid are placed into a tapering passage. Energy is applied to the cell, thereby inducing poration. To sort cells, a cellular suspension is placed in a tapering passage, including a narrow end that defines an opening that has a dimension corresponding to a cell size. An acoustic wave is applied, thereby forcing cells having a cell size smaller than the selected cell size through the opening, with a portion of the cells having a cell size not smaller than the selected cell size not forced through the opening. To extract material from a cell, an electric field and an acoustic wave are applied, thereby causing the cell membrane to allow the material to pass out of the cell.
    URI
    http://hdl.handle.net/1853/57845
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