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dc.contributor.authorAppleton, Caleb
dc.date.accessioned2017-07-28T18:32:32Z
dc.date.available2017-07-28T18:32:32Z
dc.date.created2015-05
dc.date.issued2015-06-30
dc.date.submittedMay 2015
dc.identifier.urihttp://hdl.handle.net/1853/58450
dc.description.abstractThe performed research examines the response of K562 cells to targeted introduction of double strand DNA breaks. The research provides a comparison of repair via non-homologous end joining and homology directed repair at an endogenous loci. For the experiments, a number of DNA modification techniques were utilized: transcription activator like effector nucleases, zinc finger nucleases and clustered regularly interspaced palindromic repeats. Findings showed that when given a donor template for repair, all gene modification methods showed repair by both HDR and NHEJ, however the rates were not always robust. Highest rates of gene modification were observed in paired CRISPR nickases. The study highlights the need for better understanding of gene repair strategies at endogenous loci.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherGeorgia Institute of Technology
dc.subjectTALEN
dc.subjectCRISPRS
dc.subjectZFN
dc.subjectHDR
dc.subjectNHEJ
dc.titleInvestigation of preferential DNA repair mechanisms in K562 cells as a result of targeted genome engineering
dc.typeUndergraduate Research Option Thesis
dc.description.degreeUndergraduate
dc.contributor.departmentBiomedical Engineering (Joint GT/Emory Department)
thesis.degree.levelUndergraduate
dc.contributor.committeeMemberBao, Gang
dc.contributor.committeeMemberKubanek, Julia
dc.date.updated2017-07-28T18:32:32Z


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