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dc.contributor.authorLaohapant, Alvin
dc.date.accessioned2017-07-28T18:33:42Z
dc.date.available2017-07-28T18:33:42Z
dc.date.created2017-05
dc.date.submittedMay 2017
dc.identifier.urihttp://hdl.handle.net/1853/58498
dc.description.abstractHematopoietic stem cells (HSCs) have the ability to differentiate into any blood cell as well as self-renew, giving rise to their pluripotent attribute. With the ability to differentiate, HSCs have the potential to be transplanted from healthy donors to matched patients with hematological malignancies as well as bone marrow failure. While the bulk of HSCs are located within the bone marrow, mobilization into the peripheral blood is required for accessible collection of HSCs, which ultimately eliminates the need for surgical procedures. Previous research findings have found that hematopoietic growth factor cytokines, more specifically Granulocyte colony-stimulating factor (G-CSF), as well as the mobilizing agent, plerixafor (AMD3100) increase mobilization of HSCs into the peripheral blood. While G-CSF and AMD3100 have both been scientifically proven and approved to increase HSC mobilization, the mechanical properties of HSCs have yet to be observed when mobilizing from the bone marrow to the peripheral blood. Here we use HSCs flowing through a microfluidic model to represent mobilization and hope to see cell softening due to AMD3100 during transit through the microfluidic device. By investigating the mechanical properties of HSCs during mobilization in the presence of AMD3100, clinical significance can lead to further studies as well as alternative mobilization techniques for use with HSC transplantation for patients with hematological malignancies as well as bone marrow failure.
dc.format.mimetypeapplication/pdf
dc.publisherGeorgia Institute of Technology
dc.subjectHematopoietic stem cells
dc.subjectHSC
dc.subjectHSCs
dc.subjectStem cell
dc.subjectMobilization
dc.subjectAMD3100
dc.subjectPlerixafor
dc.subjectBiophysical flow cytometry
dc.titleHematopoietic stem cell softening mediates mobilization due to AMD3100, thereby increasing count in peripheral blood
dc.typeUndergraduate Research Option Thesis
dc.description.degreeUndergraduate
dc.contributor.departmentBiomedical Engineering (Joint GT/Emory Department)
thesis.degree.levelUndergraduate
dc.contributor.committeeMemberLam, Wilbur
dc.contributor.committeeMemberHarley, Linda
dc.date.updated2017-07-28T18:33:42Z


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