• Login
    View Item 
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Structural analysis of virus assembly by cryo-electron tomography: Measles virus and respiratory syncytial virus

    Thumbnail
    View/Open
    KE-DISSERTATION-2018.pdf (153.3Mb)
    KE_Dissertation_Movie_Legend.pdf (34.76Kb)
    Movie4.1_RSV_NatImmuno.mov (47.15Mb)
    Movie3.4_RSV_Reconstructed_500nm.mov (18.13Mb)
    Movie3.3_RSV_TiltSeries_500nm.mov (7.074Mb)
    Movie3.2_RSV_Assembly_Segmentation.mov (52.24Mb)
    Movie3.1_RSV_Assembly_Stages.mov (78.05Mb)
    Movie2.12_MeV_Reconstructed_200nm.mov (52.87Mb)
    Movie2.11_MeV_TiltSeries_200nm.mov (39.52Mb)
    Movie2.10_RNP_length_200nm.mov (22.68Mb)
    Movie2.9_RNP_length_200nm.mov (26.85Mb)
    Movie2.8_RNP_clonemodel_100nm.mov (45.25Mb)
    Movie2.7_FM_Lattice_RNP.mov (20.00Mb)
    Movie2.6_Edm_FM_lattice_10nm.mov (3.069Mb)
    Movie2.5_recMeV_FM_Lattice.mov (15.68Mb)
    Movie2.4_recMeV_Assembly_F_lattice.mov (74.14Mb)
    Movie2.3_Edm_released_M_RNP.mov (18.28Mb)
    Movie2.2_Edm_MRC-5_Assembly_200nm.mov (24.22Mb)
    Movie2.1_Edm_Assembly.mov (38.44Mb)
    Date
    2018-03-27
    Author
    Ke, Zunlong
    Metadata
    Show full item record
    Abstract
    Viruses, are such efficient nano-machines that they can pack all the genomic materials in a confined space essential for self-replication; and yet they are the smallest of living entities among all life forms. When studied carefully, viruses can parasitize in all living organisms. Animals, plants, bacteria, and fungi are all subject to viral infections. In this dissertation, I will be mainly focusing on investigating measles virus (MeV) and respiratory syncytial virus (RSV) by cryo-electron tomography (cryo-ET). MeV and RSV are single-stranded negative-sense RNA viruses. They are important human pathogens that can cause severe diseases; in some cases, they can lead to death. MeV and RSV are known for their pleomorphic nature. Because of the inherent heterogeneity, the structural studies of these two viruses have not been well explored until recent years. Whole-cell tomography of virus-infected cells is possible and provides a much more native environment to study virus assembly as well as the authentic architecture of viral particles. MeV remains a major human pathogen, with recurrent outbreaks impacting the pediatric population worldwide. To elucidate the principles governing paramyxovirus assembly and budding, we used cryo-ET to directly visualize MeV-infected human-derived cells. The three-dimensional (3D) arrangement of the MeV structural proteins including the surface glycoproteins (F and H), matrix protein (M), and the ribonucleoprotein complex (RNP) were characterized at stages of virus assembly and budding, and in released virus particles. A two-layered F-M lattice was revealed and the F-M lattice suggests that interactions between these proteins are present and may coordinate processes essential for MeV assembly. In this model, the M lattice facilitates the well-ordered incorporation and concentration of the surface glycoproteins and the RNP at sites of virus assembly. RSV is the leading cause of lower respiratory tract disease in young children, immuno-compromised adults, and the elderly. In this study, we have used cryo-ET to study the morphology and assembly of RSV from infected human cells in its close-to-native state. Our results have demonstrated that RSV is filamentous across several virus strains and cell lines by cryo-ET, cryo-immuno EM, and thin sectioning TEM techniques. Taking advantage of the whole cell tomography technique, we have resolved different stages of RSV assembly. Collectively, our results will facilitate the understanding of viral morphogenesis in RSV and other pleomorphic enveloped viruses.
    URI
    http://hdl.handle.net/1853/59735
    Collections
    • Georgia Tech Theses and Dissertations [23877]
    • School of Biology Theses and Dissertations [464]

    Related items

    Showing items related by title, author, creator and subject.

    • Quantifying marine virus-host abundance relationships 

      Wigington, Charles Harrison (Georgia Institute of Technology, 2017-08-17)
      Marine microbes are the most abundant cellular life forms on Earth yet we know viruses to be even more numerous than microbes. Marine microbes are estimated to total roughly ten to the thirtieth power while marine viruses ...
    • Understanding virus-host interactions through single cell and whole genome analysis 

      Peng, Shengyun (Georgia Institute of Technology, 2018-11-07)
      Viruses and their microbial hosts are widely distributed in the environment, including in oceans, soils, fresh water, and even in extreme environments such as the deep ocean, hot springs and the upper atmosphere. Given the ...
    • An Anti-Influenza Strategy Based on siRNA Approach Targeting the Critical Viral and Host Factors 

      Liu, Timothy (Georgia Institute of Technology, 2022-05)
      The influenza virus remains a major public health concern causing significant morbidity and mortality in humans. Vaccination is considered the most effective prevention strategy as antivirals are suboptimal in treating the ...

    Browse

    All of SMARTechCommunities & CollectionsDatesAuthorsTitlesSubjectsTypesThis CollectionDatesAuthorsTitlesSubjectsTypes

    My SMARTech

    Login

    Statistics

    View Usage StatisticsView Google Analytics Statistics
    facebook instagram twitter youtube
    • My Account
    • Contact us
    • Directory
    • Campus Map
    • Support/Give
    • Library Accessibility
      • About SMARTech
      • SMARTech Terms of Use
    Georgia Tech Library266 4th Street NW, Atlanta, GA 30332
    404.894.4500
    • Emergency Information
    • Legal and Privacy Information
    • Human Trafficking Notice
    • Accessibility
    • Accountability
    • Accreditation
    • Employment
    © 2020 Georgia Institute of Technology