• Login
    View Item 
    •   SMARTech Home
    • Undergraduate Research Opportunities Program (UROP)
    • Undergraduate Research Option Theses
    • View Item
    •   SMARTech Home
    • Undergraduate Research Opportunities Program (UROP)
    • Undergraduate Research Option Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Cellular Characterization of Microvascular Fragments and Stromal Vascular Fraction for the Treatment of Composite Bone-Muscle Defects

    Thumbnail
    View/Open
    WANG-UNDERGRADUATERESEARCHOPTIONTHESIS-2017.pdf (363.5Kb)
    Date
    2017-05
    Author
    Wang, Yuyan
    Metadata
    Show full item record
    Abstract
    The current treatment for critical-sized boned defects, bone morphogenetic protein 2 (BMP-2) loaded collagen sponge grafting, is often inadequate for composite bone-muscle defects, which heal more slowly with higher rates of non- or malunion. Due the additional muscle defect, there is a vacancy of vasculature around the bone defect, causing insufficient nutrient and cytokine delivery and thus hindering bone regeneration. Hypothesizing that increasing angiogenesis will improve regeneration, we have previously proposed to co-deliver microvascular fragments (MVF) or stromal vascular fraction (SVF), which have been shown to mature into microvascular networks in vitro and in vivo, with BMP-2 in a collagen sponge. However, in preliminary studies, collagen sponge co-loaded with BMP-2 and MVF showed augmented release of BMP-2 compared to those co-loaded with SVF or with BMP-2 only. Although they are both derived from the same adipose tissue source, we hypothesize that their cellular composition may differ and thus affect BMP-2 kinetics. Therefore, this project aims to characterize the cellular components of MVF and SVF using flow cytometry to elucidate the potential mechanism of differential BMP-2 release. It was found that MVF has a relatively lower percentage of mesenchymal stem cells (MSCs) and relatively higher percentage of mature endothelial cells (ECs) than SVF, suggesting a role for ECs in the augmented BMP-2 release from MVF-loaded collagen sponges.
    URI
    http://hdl.handle.net/1853/60336
    Collections
    • Department of Biomedical Engineering Undergraduate Research Option Theses [214]
    • Undergraduate Research Option Theses [862]

    Browse

    All of SMARTechCommunities & CollectionsDatesAuthorsTitlesSubjectsTypesThis CollectionDatesAuthorsTitlesSubjectsTypes

    My SMARTech

    Login

    Statistics

    View Usage StatisticsView Google Analytics Statistics
    facebook instagram twitter youtube
    • My Account
    • Contact us
    • Directory
    • Campus Map
    • Support/Give
    • Library Accessibility
      • About SMARTech
      • SMARTech Terms of Use
    Georgia Tech Library266 4th Street NW, Atlanta, GA 30332
    404.894.4500
    • Emergency Information
    • Legal and Privacy Information
    • Human Trafficking Notice
    • Accessibility
    • Accountability
    • Accreditation
    • Employment
    © 2020 Georgia Institute of Technology