Potential therapy against Glioblastoma using Motile Nanocarrier Expressing TRAIL and Azurin

Abstract

Glioblastoma (GBM) is the most common and aggressive type of gliomas in humans. It is also highly resistant to most treatment methods. Nearly 12,000 people are diagnosed with glioblastoma each year in the United States. Approximately two out of three glioblastoma patients will fail today’s approved therapies. The tumor grows by changing normal brain cells to stem cell, which can continuously replicate and regrow a tumor with only a handful of cells left behind. In this paper we research two different methods to approach the problem. The first method 1) Cloning soluble TRAIL into plasmid with hypoxic promoter and transforming into avirulent Salmonella Typhimurium for inducing apoptosis in Glioblastoma cells and the second method is 2) To check if avirulent Salmonella Typhirium seeks tumors and expresses proteins such as Azurin. This paper demonstrates the qualitative results of both.