• Login
    View Item 
    •   SMARTech Home
    • Undergraduate Research Opportunities Program (UROP)
    • Undergraduate Research Option Theses
    • View Item
    •   SMARTech Home
    • Undergraduate Research Opportunities Program (UROP)
    • Undergraduate Research Option Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Characterizing iron reducing microorganisms from anoxic ferruginous lake sediments

    Thumbnail
    View/Open
    COSTA-UNDERGRADUATERESEARCHOPTIONTHESIS-2018.pdf (13.03Mb)
    Author
    Costa, Bianca Francesca
    Metadata
    Show full item record
    Abstract
    Lake Matano, Indonesia is a well-known ancient ocean analogue as its anoxic ecosystem in the subsurface sediments allow the growth of microorganisms capable of mediating anaerobic oxidation coupled to iron reduction. An anoxic, ferric iron rich BSR was kept for 163 days to culture iron reducing bacteria in an environmental sample from Lake Matano sediment. Microscopy analysis was performed periodically to look for morphology trends using DAPI staining. Phylogenetic analysis was performed using CARD-FISH microscopy to target the Geobacter genus, known for electroactive abilities through pilA and interactions with iron particles. Cell counts correlated with iron reduction throughout the culturing period indicating iron reducing microorganisms were present in the environmental sample. Filamentous, coccus, bacillus and vibrio morphologies were observed through the culturing period with vibrio and bacillus being predominant at later stages of the culture. Particle association instances were observed at days 20, 62, 86 and 126 and DIET interactions at day 86. Characterizing these microorganisms opens a broad range of possibilities for wastewater treatment (strip mining groundwater contamination), use in bioelectrochemical systems biofilms (due to external electron transport pilA) and understanding the importance of this microorganisms in Iron cycling.
    URI
    http://hdl.handle.net/1853/60891
    Collections
    • School of Earth and Atmospheric Sciences Undergraduate Research Option Theses [10]
    • Undergraduate Research Option Theses [756]

    Browse

    All of SMARTechCommunities & CollectionsDatesAuthorsTitlesSubjectsTypesThis CollectionDatesAuthorsTitlesSubjectsTypes

    My SMARTech

    Login

    Statistics

    View Usage StatisticsView Google Analytics Statistics
    facebook instagram twitter youtube
    • My Account
    • Contact us
    • Directory
    • Campus Map
    • Support/Give
    • Library Accessibility
      • About SMARTech
      • SMARTech Terms of Use
    Georgia Tech Library266 4th Street NW, Atlanta, GA 30332
    404.894.4500
    • Emergency Information
    • Legal and Privacy Information
    • Human Trafficking Notice
    • Accessibility
    • Accountability
    • Accreditation
    • Employment
    © 2020 Georgia Institute of Technology