Characterization of the electrophysiology of neurons in the central nervous system of Ciona robusta
Martinez - Feduchi Guijo, Paula
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The objective of this study was to investigate the electrophysiological properties of C. robusta motor ganglion neurons—interneuron 2 (IN2) and the descending decussating neuron (ddN)— and the sensory cells of the papillae (Axial Columnar Cells, or ACCs). Primary cell cultures of mid-tailbud C. robusta larvae 24 hours after dissociation exhibited cell death and cell clumping, as well as neurons with short axons, typical of invertebrate larval neurons. In addition, cells did not attach to the poly-L-lysine glass coverslips. Improvements to the dissociation protocol reduced environmental stress and increased cell viability in plastic petri dishes only, with cells continuing to exhibit cell death when plated onto the poly-L-lysine coated glass coverslips. Future experiments should focus on refining the primary cell culture protocols to increase cell viability and cell attachment, as well as decreasing bacterial contamination.