• Login
    View Item 
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    •   SMARTech Home
    • Georgia Tech Theses and Dissertations
    • Georgia Tech Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Analysis of post translational modifications in the regulation of cell signaling and behavior

    Thumbnail
    View/Open
    CHOUDHURY-DISSERTATION-2018.pdf (4.756Mb)
    Date
    2018-07-25
    Author
    Choudhury, Shilpa
    Metadata
    Show full item record
    Abstract
    Post-translational modifications (PTMs) are chemical or proteinaceous modifications that occur on an expressed protein and can influence protein structure, stability, interaction, and localization. Thus, PTMs represent a mode of proteomic control that is fast, versatile, and dynamic; and regulate a plethora of biological processes, including cell signaling, cellular proliferation and differentiation, cell adhesion and migration, and many more. In this thesis, my aim was to understand the role of PTMs in the regulation of two diverse physiological processes- G protein signaling (primary doctoral project) and extracellular matrix mediated cell adhesion and migration (collaborative project with Professor Thomas Barker, University of Virginia). The first part of the thesis discusses PTM mediated regulation of G-protein signaling, for which I have discovered novel regulatory properties of phosphorylation sites in the N-terminal tails of G gamma subunits. Through a series of biochemical and cellular assays, I showed that phosphorylated G gamma and G beta gamma effector protein, together control feedback mediated regulation of MAPK activation. The second part of the thesis discusses how I have employed mass spectrometry based characterization of citrullination, a rare and understudied PTM that is known to occur on extracellular matrix proteins, such as fibronectin, and disrupt cell adhesion and migration signaling in humans. My hope is that the discoveries made in this thesis will not only further our understanding of PTM mediated regulation of G-protein, but also open avenues for future studies in the field of extracellular matrix biology.
    URI
    http://hdl.handle.net/1853/61653
    Collections
    • Georgia Tech Theses and Dissertations [23877]
    • School of Biology Theses and Dissertations [464]

    Browse

    All of SMARTechCommunities & CollectionsDatesAuthorsTitlesSubjectsTypesThis CollectionDatesAuthorsTitlesSubjectsTypes

    My SMARTech

    Login

    Statistics

    View Usage StatisticsView Google Analytics Statistics
    facebook instagram twitter youtube
    • My Account
    • Contact us
    • Directory
    • Campus Map
    • Support/Give
    • Library Accessibility
      • About SMARTech
      • SMARTech Terms of Use
    Georgia Tech Library266 4th Street NW, Atlanta, GA 30332
    404.894.4500
    • Emergency Information
    • Legal and Privacy Information
    • Human Trafficking Notice
    • Accessibility
    • Accountability
    • Accreditation
    • Employment
    © 2020 Georgia Institute of Technology