Ability of Janus Particle as Single Cell Sensor to Immobilize Plasma Cells and Collect Antibody
Lee, Ye Lim
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The method to identify and isolate the proteins is one of the keys to the immunoassay production. However, currently there is no effective way to isolate the protein not only because of limited number of technologies for protein detection but also the existing technologies have downfall of low sensitivity for protein detection and the secreted protein cross-reactivity. The present paper demonstrates the isolation and collection ability of the Janus particle. The study demonstrates the creation of Janus particle with one side of the particle targets and binds plasma cells while the other side contains protein G that collects specific antibodies that are secreted. Experiments were conducted to determine the ability of 2 μm and 4 μm size Janus Particle. Moreover, the study was carried out with hybridoma cells to determine whether the Janus particle can bind to the plasma B cell and collect antibody with another hemisphere.