An investigation of the mechanism behind Adjuplex and its effect in adjuvant combinations
Vogel, Gabriela Maria
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This research thesis contributes to the field of biomedical engineering, specifically to the following topics: immunotherapy, drug delivery, and adjuvant-vaccine work. By utilizing RAW-Blue cells to observe IFN-β production, NF-κB production, and overall activation of TLRs, we were able to show the effects dosage and combinations of adjuvants have on immune activation. While investigations summarized in this thesis show that Adjuplex in combination with other common adjuvants such as MPLA and CpG decreases the production of IFN-β, this did not indicate that Adjuplex suppresses the immune response that known adjuvants normally induce. Additionally, Luminex assays, ELISA assays, and flow cytometry experiments were performed to learn more about the mechanism behind Adjuplex’s ability to produce an immune response and more in depth on how Adjuplex interacts with other adjuvants in combination. Results showed that there was synergy between Adjuplex and GLA when looking at cytokines produced through the inflammasome pathway. Therefore, future work should expand on this finding and experiments should focus on further validating this finding by looking at other markers in the inflammasome pathway. Characterization of the type of adaptive immune response Adjuplex produces as an adjuvant is of critical relevance to the development of synergistic or suppressive adjuvant combinations and the optimization of vaccines.