Show simple item record

dc.contributor.advisorSnell, Terry
dc.contributor.authorBrashear, Jillian
dc.date.accessioned2021-06-30T17:37:27Z
dc.date.available2021-06-30T17:37:27Z
dc.date.created2021-05
dc.date.submittedMay 2021
dc.identifier.urihttp://hdl.handle.net/1853/64854
dc.description.abstractRotifers are sensitive indicators of environmental conditions and serve as model organisms for assessing toxicity. It is understood that rotifer cysts (diapausing eggs) are convenient for toxicity assessment because they remove the need to maintain animal cultures, reduce variability in tests, and can be stored for on-demand use. Indeed, cyst-based toxicity tests for the rotifer Proales similis have helped to fill a need for an additional marine animal model to the rotifer Brachionus plicatilis in ecotoxicology. A challenge to implementing tests is the need for readily- available P. similis diapausing eggs, which need to be reliably preserved and hatched on demand. This study explores preservation methods to extend the viability of P. similis eggs. We explore factors including storage temperature, salinity, and the addition of ascorbic acid to measure their effects independently and combined. We found that storing the diapausing eggs at approximately 4℃ in the presence of 20µM ascorbic acid and a 220ppt salinity is effective for extending egg viability. With longer viability, P. similis diapausing eggs are more readily available and thereby valuable as a tool in toxicity assessments.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherGeorgia Institute of Technology
dc.subjectRotifer
dc.subjectDiapausing eggs
dc.titleUSING ASCORBIC ACID AND HIGH SALINITY TO EXTEND THE VIABILITY OF PROALES SIMILIS (ROTIFERA) DIAPAUSING EGGS
dc.typeUndergraduate Research Option Thesis
dc.description.degreeUndergraduate
dc.contributor.departmentBiology
thesis.degree.levelUndergraduate
dc.contributor.committeeMemberWeigel , Emily
dc.contributor.committeeMemberGoodisman , Michael
dc.date.updated2021-06-30T17:37:28Z


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record