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dc.contributor.advisorReddi, Amit
dc.contributor.authorHale, Owen Francis
dc.date.accessioned2021-06-30T17:37:35Z
dc.date.available2021-06-30T17:37:35Z
dc.date.created2021-05
dc.date.submittedMay 2021
dc.identifier.urihttp://hdl.handle.net/1853/64861
dc.description.abstractMycobacterium tuberculosis is responsible for more human deaths every year than any other bacterium. In order to establish an infection and cause disease, M. tuberculosis requires the nutrient heme. Heme is essential for numerous processes within the cell but is also cytotoxic, so its synthesis, uptake, and utilization must be tightly regulated by the bacterium. Despite the important role of this nutrient, relatively little is known about the regulation of its abundance and bioavailability. To better understand heme dynamics in mycobacteria, a total heme fluorescence assay and genetically encoded exchange labile heme sensors were used to observe changes in the abundance and bioavailability of heme in Mycobacterium smegmatis under various conditions. These experiments demonstrate that heme abundance and bioavailability in mycobacteria can change in response to physiologically relevant stresses and environmental cues.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherGeorgia Institute of Technology
dc.subjectMycobacteria
dc.subjectMycobacterium smegmatis
dc.subjectHeme
dc.subjectMicrobiology
dc.titleProbing the Dynamic Nature of Mycobacterial Heme Homeostasis
dc.typeUndergraduate Research Option Thesis
dc.description.degreeUndergraduate
dc.contributor.departmentBiology
thesis.degree.levelUndergraduate
dc.contributor.committeeMemberWhiteley, Marvin
dc.date.updated2021-06-30T17:37:35Z


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