Sleep Disruption in a Mouse Model of Medial Temporal Lobe Epilepsy
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While not typically a recognized comorbidity of epilepsy, sleep disorders are common in epilepsy, particularly medial temporal lobe epilepsy (MTLE). However, consistent and severe sleep disruptions have a potentially cascading effect in the severity of seizure control, as well as other comorbidities of TLE. The present study uses the intra-amygdala kainic acid (IAKA) mouse model of TLE to develop a basis for novel sleep-focused treatments. Using a custom 3-D printed headset equipped with electroencephalogram (EEG) electrodes, electromyogram (EMG) electrodes, and a guide cannula, mice were injected with kainic acid targeting the left basolateral amygdala and were recorded continuously throughout the duration of the experiment. Preliminary results show that IAKA mice (with seizures) spent more time in wake and less time in non-rapid eye movement (NREM) and rapid eye movement (REM) sleep over the experiment’s final 72 hours, compared to controls. Furthermore, a weak positive correlation was identified between percent of time spent in wake and amount of seizures. Histological data showed amygdala and hippocampal damage in mice with seizures.