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dc.contributor.authorMouw, Janna Kayen_US
dc.date.accessioned2006-01-18T22:22:28Z
dc.date.available2006-01-18T22:22:28Z
dc.date.issued2005-11-28en_US
dc.identifier.urihttp://hdl.handle.net/1853/7550
dc.description.abstractIn pathological states such as osteoarthritis, the complex metabolic balance of cartilage is disrupted, leading to a loss in the integrity and biomechanical function of cartilage. Osteoarthritis affects more than 20 million Americans, costing the United States economy over $60 billion yearly. Risk factors for osteoarthritis include age, excessive joint loading, and joint injury. Tissue engineering offers a potential solution for the replacement of diseased and/or damaged cartilage. Unfortunately, plentiful donor cell populations are difficult to assemble, as chondrocytes have a well characterized lack of expansion potential. Mesenchymal progenitor cells offer an alternative with a high expansion potential capable of supplying large quantities of cells. Using an immature bovine model, the chondrogenic differentiation of articular chondrocytes and bone marrow stromal cells was found to be scaffold, media and mechanical stimulation dependent. TGF-beta signaling participated in the response of articular chondrocytes to dynamic compressive loading, as well as enhanced the chondrogenesis of bovine BMSCs, through interactions between loading and TGF-beta/Smad signaling. Also, dynamic loading altered gene expression, matrix synthesis rates and intracellular phosphorylation for bovine BMSCs. However the response of the cells to dynamic loading depends on both media supplementation and the duration of unloaded culture. These studies establish signaling through the TGF-beta pathway as a mechanotransduction pathway for chondrocytes and chondroprogenitors in 3D culture.en_US
dc.format.extent1750505 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherGeorgia Institute of Technologyen_US
dc.subjectChondrogenesisen_US
dc.subjectChondrocyte
dc.subjectStromal cells
dc.subject.lcshCell differentiationen_US
dc.subject.lcshTissue engineeringen_US
dc.subject.lcshArticular cartilageen_US
dc.subject.lcshBiomechanicsen_US
dc.subject.lcshCartilage cellsen_US
dc.titleMechanoregulation of chondrocytes and chondroprogenitors: the role of TGF-BETA and SMAD signalingen_US
dc.typeDissertationen_US
dc.description.degreePh.D.en_US
dc.contributor.departmentBioengineeringen_US
dc.description.advisorCommittee Chair: Marc E. Levenston; Committee Member: Andres Garcia; Committee Member: Barbara Boyan; Committee Member: Christopher Jacobs; Committee Member: Harish Radhakrishnaen_US


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