Replication-Dependent Mechanism of Chromosome Fragility at the Site of Inverted Repeats in Saccharomyces cerevisiae

Show full item record

Please use this identifier to cite or link to this item: http://hdl.handle.net/1853/13579

Title: Replication-Dependent Mechanism of Chromosome Fragility at the Site of Inverted Repeats in Saccharomyces cerevisiae
Author: Moon, Clara
Abstract: Genome instability is linked to cancer and many hereditary diseases. Chromosomal aberrations are often associated with repeats that can adopt DNA secondary structures. Studying the mechanism of genome instability caused by unstable motifs will therefore contribute to our understanding of the origin of human pathology. In this study, we investigate the mechanism underlying inverted repeated associated instability. A biological assay was used to identify mutant strains that showed increased instability in the presence of inverted repeats in the ∆sae2 or ∆mre11 background. Tetrad analysis techniques were used to map the mutations, and four mutations (all isolated independently) were identified in the CDC2 (POL3) gene. We focused on one mutation, pol3-6PL for this study. The effect of pol3-6PL on inverted repeat-mediated instability was assessed using armloss and gene amplification assays. Fluctuation tests on the mutant strain showed increased rates of these events. CHEF and Southern blot hybridization showed increased double-strand break formation at the site of inverted repeats. From this data, we propose a model for a replication-dependent mechanism of chromosomal fragility at the site of inverted repeats.
Type: Undergraduate Thesis
URI: http://hdl.handle.net/1853/13579
Date: 2006-12-05
Publisher: Georgia Institute of Technology
Subject: Chromosome fragility
Cruciform structures
Double-strand breaks
Hairpin structures
Inverted repeats
Mre11/Rad50/Xrs2
POL3
Sae2
Department: Biology
Advisor: Advisor: Dr. Kirill Lobachev ; Reader: Dr. Yury Chernoff

All materials in SMARTech are protected under U.S. Copyright Law and all rights are reserved, unless otherwise specifically indicated on or in the materials.

Files in this item

Files Size Format View
Moon_Clara_200612_ro.pdf 574.1Kb PDF View/ Open

This item appears in the following Collection(s)

Show full item record