Arterial Response to Local Mechanical Variables: The Effects of Circumferential and Shear Stress

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1853/22611

Title: Arterial Response to Local Mechanical Variables: The Effects of Circumferential and Shear Stress
Author: Wayman, Brian H.
Abstract: Arteries respond to changes in global mechanical parameters (pressure, flow rate, and longitudinal stretching) by remodeling to restore local parameters (circumferential stress, shear stress, and axial strain) to baseline levels. Because a change in a single global parameter results in changes of multiple local parameters, the effects of individual local parameters on remodeling remain unknown. This study uses a novel approach to study remodeling in organ culture based on independent control of local mechanical parameters. The approach is illustrated by studying the effects of circumferential and shear stress on remodeling-related biological markers. Porcine carotid arteries were cultured for three days at a circumferential stress of 50 kPa or 150 kPa or, in separate experiments, a shear stress of 0.75 Pa or 2.25 Pa. At high circumferential stress, matrix synthesis, smooth muscle cell proliferation, and cell death are significantly greater, but matrix metalloproteinase-2 (MMP-2) and pro-MMP-2 activity are significantly less. In contrast, biological markers measured were unaffected by shear stress. Applications of the proposed approach for improved understanding of remodeling, optimizing mechanical conditioning of tissue engineered arteries, and selection of experimentally motivated growth laws are discussed.
Type: Dissertation
URI: http://hdl.handle.net/1853/22611
Date: 2007-04-09
Publisher: Georgia Institute of Technology
Subject: Carotid artery
Vasomotor response
Biological markers
Remodeling
Arteries
Blood flow
Hemodynamics
Shear flow
Organ culture
Tissue remodeling
Mathematical models
Department: Mechanical Engineering
Advisor: Committee Chair: Vito, Raymond; Committee Member: Guldberg, Robert; Committee Member: Levenston, Marc; Committee Member: McDevitt, Todd; Committee Member: Rachev, Alexander
Degree: Ph.D.

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