The Two Unknowns of Nucleosomes: How They Are Formed and How They Are Removed

Show simple item record Kim, Harold 2012-04-03T20:54:04Z 2012-04-03T20:54:04Z 2012-03-13
dc.description Presented on March 13, 2012 from 8:30 a.m.-9:30 a.m. at the Parker H. Petit Institute for Bioengineering & Bioscience (IBB), room 1128, Georgia Tech. en_US
dc.description Runtime: 60:19 minutes
dc.description.abstract In eukaryotes, the genomic DNA is highly packaged inside the nucleus of a cell by forming beads-on-a string-like structure called nucleosomes. The propensity of a ~150-bp duplex DNA to form a nucleosome, termed the "nucleosome affinity", varies over a few orders of magnitude depending on the DNA sequence. Although nucleosome affinity is thought to be determined by DNA bending stiffness, the exact relationship between the two is not clear. Besides their role in DNA packaging, nucleosomes can repress gene expression by preventing transcription factors from accessing their DNA binding sites. Some nucleosomes, however, do not directly occlude transcription factor binding sites, and therefore their role in gene expression remains unknown. In the first part of this talk, I will explain how we measure looping kinetics and permanent curvature of various sequences of DNA using single-molecule FRET (fluorescence resonance energy transfer) and gel electrophoresis. I will then estimate nucleosome affinity of different sequences based on these measurements. In the second part, I will explain how we quantify gene expression pattern using fluorescence microscopy of yeast cells and demonstrate two examples where a nucleosome exhibits opposite effects on gene expression. Based on these results, I will present a model for nucleosome removal prior to transcription initiation. en_US
dc.format.extent 60:19 minutes
dc.language.iso en_US en_US
dc.publisher Georgia Institute of Technology en_US
dc.relation.ispartofseries Petit Institute Breakfast Club Seminar Series
dc.subject Biophysics en_US
dc.subject Genomics en_US
dc.title The Two Unknowns of Nucleosomes: How They Are Formed and How They Are Removed en_US
dc.type Lecture en_US
dc.type Video en_US
dc.contributor.corporatename Georgia Institute of Technology. Institute for Bioengineering and Bioscience
dc.contributor.corporatename Georgia Institute of Technology. School of Physics

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